Rapid diagnosis of human brucellosis by peripheral-blood PCR assay
Copyright policy )Rapid diagnosis of human brucellosis by peripheral-blood PCR assay
A single-step PCR assay with genus-specific primers for the amplification of a 223-bp region of the sequence encoding a 31-kDa immunogenetic Brucella abortus protein (BCSP31) was used for the rapid diagnosis of human brucellosis. We examined peripheral blood from 47 patients, with a total of 50 cases of brucellosis, and a group of 60 control subjects, composed of patients with febrile syndromes of several etiologies other than brucellosis, asymptomatic subjects seropositive for Brucella antibodies, and healthy subjects. Diagnosis of brucellosis was established in 35 cases (70%) by isolation of Brucella in blood culture and in the other 15 cases (30%) by clinical and serological means. The sensitivity of our PCR assay was 100%, since it correctly identified all 50 cases of brucellosis, regardless of the duration of the disease, the positivity of the blood culture, or the presence of focal forms. The specificity of the test was 98.3%, and the only false-positive result was for a patient who had had brucellosis 2 months before and possibly had a self-limited relapse. In those patients who relapsed, the results of our PCR assay were positive for both the initial infection and the relapse, becoming negative once the relapse treatment was completed and remaining negative in the follow-up tests at 2, 4, and 6 months. In conclusion, these results suggest that the PCR assay is rapid and easy to perform and highly sensitive and specific, and it may therefore be considered a useful tool for diagnosis of human brucellosis.
- University of Malaga Spain
Adult, DNA, Bacterial, Male, Medical Subject Headings::Diseases::Bacterial Infections and Mycoses::Bacterial Infections::Gram-Negative Bacterial Infections::Brucellosis, Medical Subject Headings::Named Groups::Persons::Age Groups::Adult::Aged::Aged, 80 and over, Adolescent, Fever, Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Genetic Techniques::Nucleic Acid Amplification Techniques::Polymerase Chain Reaction, Brucella abortus, Medical Subject Headings::Named Groups::Persons::Age Groups::Adult::Aged, Medical Subject Headings::Diseases::Pathological Conditions, Signs and Symptoms::Signs and Symptoms::Body Temperature Changes::Fever, Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Epidemiologic Methods::Epidemiologic Research Design::Sensitivity and Specificity, Polymerase Chain Reaction, Sensitivity and Specificity, Brucellosis, Medical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans, Reference Values, Medical Subject Headings::Named Groups::Persons::Age Groups::Adult, Humans, Medical Subject Headings::Chemicals and Drugs::Nucleic Acids, Nucleotides, and Nucleosides::Nucleic Acids::DNA::DNA, Bacterial, Medical Subject Headings::Named Groups::Persons::Age Groups::Adolescent, Medical Subject Headings::Named Groups::Persons::Age Groups::Adult::Middle Aged, Aged, DNA Primers, Aged, 80 and over, Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Globulins::Serum Globulins::Immunoglobulins::Antibodies::Antibodies, Bacterial, Medical Subject Headings::Organisms::Bacteria::Gram-Negative Bacteria::Gram-Negative Aerobic Bacteria::Gram-Negative Aerobic Rods and Cocci::Brucellaceae::Brucella::Brucella abortus, Reproducibility of Results, Medical Subject Headings::Check Tags::Female, Middle Aged, Antibodies, Bacterial, Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Evaluation Studies as Topic::Reproducibility of Results, Medical Subject Headings::Check Tags::Male, Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Weights and Measures::Reference Values, Female, Medical Subject Headings::Chemicals and Drugs::Chemical Actions and Uses::Specialty Uses of Chemicals::Laboratory Chemicals::Molecular Probes::Nucleic Acid Probes::DNA Probes::DNA Primers
Adult, DNA, Bacterial, Male, Medical Subject Headings::Diseases::Bacterial Infections and Mycoses::Bacterial Infections::Gram-Negative Bacterial Infections::Brucellosis, Medical Subject Headings::Named Groups::Persons::Age Groups::Adult::Aged::Aged, 80 and over, Adolescent, Fever, Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Genetic Techniques::Nucleic Acid Amplification Techniques::Polymerase Chain Reaction, Brucella abortus, Medical Subject Headings::Named Groups::Persons::Age Groups::Adult::Aged, Medical Subject Headings::Diseases::Pathological Conditions, Signs and Symptoms::Signs and Symptoms::Body Temperature Changes::Fever, Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Epidemiologic Methods::Epidemiologic Research Design::Sensitivity and Specificity, Polymerase Chain Reaction, Sensitivity and Specificity, Brucellosis, Medical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans, Reference Values, Medical Subject Headings::Named Groups::Persons::Age Groups::Adult, Humans, Medical Subject Headings::Chemicals and Drugs::Nucleic Acids, Nucleotides, and Nucleosides::Nucleic Acids::DNA::DNA, Bacterial, Medical Subject Headings::Named Groups::Persons::Age Groups::Adolescent, Medical Subject Headings::Named Groups::Persons::Age Groups::Adult::Middle Aged, Aged, DNA Primers, Aged, 80 and over, Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Globulins::Serum Globulins::Immunoglobulins::Antibodies::Antibodies, Bacterial, Medical Subject Headings::Organisms::Bacteria::Gram-Negative Bacteria::Gram-Negative Aerobic Bacteria::Gram-Negative Aerobic Rods and Cocci::Brucellaceae::Brucella::Brucella abortus, Reproducibility of Results, Medical Subject Headings::Check Tags::Female, Middle Aged, Antibodies, Bacterial, Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Evaluation Studies as Topic::Reproducibility of Results, Medical Subject Headings::Check Tags::Male, Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Weights and Measures::Reference Values, Female, Medical Subject Headings::Chemicals and Drugs::Chemical Actions and Uses::Specialty Uses of Chemicals::Laboratory Chemicals::Molecular Probes::Nucleic Acid Probes::DNA Probes::DNA Primers
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