The results of phytopathological research of cultural conifers in PetrSU Botanic Garden are described. The trees with symptoms of damage on the needles by various fungal pathogens (spotting, necrosis, drying) were analyzed. Wet chamber methods, isolation on a nutrient medium, microcopying and morphometry, as well as molecular genetic methods for the diagnosis of microorganisms were used in laboratory studies. As result some species of fungi with varying degrees of pathogenicity were identified. These researches have shown the susceptibility of cultural conifer species to most diseases. The most common pathogen is pine-leaf cast (Lophodermium pinastri Chev). Some fungi such as Hendersonia pinicola Wehm., Pestalotia sp., Cyclaneusma minus (Button) Di Cosmo were found only on young trees of cedar dwarf (Pinus pumila (Pall.) Regel).