Cytotoxicity and genotoxicity of a trypanocidal drug quinapyramine sulfate loaded-sodium alginate nanoparticles in mammalian cells
Copyright policy )Cytotoxicity and genotoxicity of a trypanocidal drug quinapyramine sulfate loaded-sodium alginate nanoparticles in mammalian cells
We synthesized quinapyramine sulfate loaded-sodium alginate nanoparticles (QS-NPs) to reduce undesirable toxic effects of QS against the parasite Trypanosoma evansi, a causative agent of trypanosomosis. To determine the safety of the formulated nanoparticles, biocompatibility of QS-NPs was determined using Vero, Hela cell lines and horse erythrocytes in a dose-dependent manner. Our experiments unveiled a concentration-dependent safety/cytotoxicity (metabolic activity), genotoxicity (DNA damage, chromosomal aberrations), production of reactive oxygen species and hemolysis in QS-NPs treated cells. Annexin-V propidium iodide (PI) staining showed no massive apoptosis or necrosis. However, at very high doses (more than 300 times than the effective doses), we observed more toxicity in QS-NPs treated cells as compared to QS treated cells. QS-NPs were safe at effective trypanocidal doses and even at doses several times higher than the effective dose.
Chromosome Aberrations, Drug Carriers, Erythrocytes, Dose-Response Relationship, Drug, Alginates, Cell Survival, Drug Compounding, Hexuronic Acids, Quinolinium Compounds, Hemolysis, Trypanocidal Agents, Glucuronic Acid, Chlorocebus aethiops, Animals, Humans, Nanoparticles, Horses, Reactive Oxygen Species, DNA Damage, HeLa Cells
Chromosome Aberrations, Drug Carriers, Erythrocytes, Dose-Response Relationship, Drug, Alginates, Cell Survival, Drug Compounding, Hexuronic Acids, Quinolinium Compounds, Hemolysis, Trypanocidal Agents, Glucuronic Acid, Chlorocebus aethiops, Animals, Humans, Nanoparticles, Horses, Reactive Oxygen Species, DNA Damage, HeLa Cells
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