Methods of biotechnology allow to obtain high-quality medicinal plant raw materials in a short time, in large quantities without destroying natural reserves. Biotechnological approaches such as aeroponic technologies have the potential for large-scale cultivation of iris plants and production of secondary metabolites. Microclonal reproduction makes it possible to obtain a healthy planting material in the required amount, regardless of the time of year. The combination of these two technological approaches will allow to develop biotechnology of year-round production of medicinal plant raw materials of Siberian iris. The study determined the content of 6-benzylaminopurine on the stage actually micropropagation for the formation of the greatest number of adventitious shoots of optimal length. The required content of BAP in the nutrient medium for I. sibirica was 2.5–5.0 µM. The introduction of cytokinins in the nutrient medium together with auxins, L-glutamine and adenine sulfate 100 mg/l, as well as the alternation of low and high concentrations of cytokinin enhanced the regenerative effect of BAP. With year-round cultivation of regenerative plants in aeroponic conditions, the amount of biomass of plant raw materials I. sibirica for this method was about 31.2 kg / m2 of crude weight in one year. It is established that intact plants and regenerative plants I. sibirica, obtained on the basis of the developed biotechnology, had identical group composition of biologically active substances. It is revealed that the sum of flavonoids in the leaves of hydroponic iris plants exceeded the content in the leaves of intact plants by 3 times, and the content of essential oil in regenerate plants and hydroponic leaves of the Sterch variety but higher by 26% compared with the leaves of intact plants. Aqueous and alcoholic extracts of I. sibirica showed antiviral activity against herpes virus. With low toxicity, both intact plants and regenerative plants had a relatively high selectivity index.