The deep processing of peat is one of the important resources for the development of the Northern Russia territories. The humic products are the most perspective from peat bio-active derivatives. At the same time, the presence of accompanying components with biological activity in them does not take into account. This research is focused on the elimination of this inaccuracy by studying the biological activity of fractions extracted from high-moor peat by the method of sequential disassembly. Bioactivity was characterized by the method of laboratory phytotesting on lettuce seeds, pretreated with solutions of preparations with stabilized pH and the content of nutrient salts. The performed studies confirmed the authors' hypothesis about the influence of the method of obtaining peat humic preparations on the biological activity. Antagonism of the effect on the phytoactivity of humic compounds, resins and waxes was revealed. The additional studies are required for an explanation of this mechanism. The fulvic acids stimulate seed germination, as part of the humic part, but intensify their damage by rot, while humic acids provide a smaller, but more stable, increase in germination due to the greater preservation of seedlings. Seed treatment with bituminous preparations inhibits the development of rot. The greatest fungicidal effect is shown by the fraction of resins, whereas peat wax, to a lesser extent, inhibits seed damage, but provides the highest and most stable stimulating effect in relation to their germination. Thus, peat bitumen waxes can serve as a good protective and stimulating agent and can be used, for example, to encapsulate seeds, as a presowing treatment to increase yields.