PCR-RFLP method in combination with on-chip electrophoresis as a tool for determining of variability between important species of Aspergillus
Copyright policy )PCR-RFLP method in combination with on-chip electrophoresis as a tool for determining of variability between important species of Aspergillus
Aspergillus species are among the most significant producers of aflatoxins, which can contaminate a wide range of agricultural and food products at any stage of production. The aim of this research was to utilize molecular methods to determine and characterize the variability between isolates of standard Aspergillus species. Genomic DNA was isolated from the mycelium of all tested Aspergillus isolates. PCR amplifications were performed using gene-specific primers. The PCR method successfully amplified the ITS1-5.8S rDNA-ITS2 region and portions of the b-tubulin and calmodulin genes of all tested Aspergillus isolates. PCR products obtained after amplification with primer pairs (ITS1/ITS4 and Bt2a/Bt2b), followed by digestion with restriction enzymes HhaI, MwoI, and AlwI in RFLP analysis, facilitated the identification of variability among the studied Aspergillus species. The results of PCR-RFLP analysis on the tested isolates were consistent with those previously obtained through morphological examinations, indicating the effectiveness of this molecular method for identification and determination of variability among important Aspergillus species. The presented molecular method based on PCR-RFLP analysis, due to its advantages such as reproducibility, speed, and high sensitivity, represents a valuable tool for monitoring and controlling contamination by Aspergillus species in the food supply chain. The method described in this study can be successfully used for rapid identification and determination of variability between isolates of Aspergillus species, contributing to improved food safety control and public health.
- Educons University Serbia
- University of Novi Sad, Institute of Food Technology Serbia
- University of Novi Sad Serbia
aspergillus species, internal transcribed spacer, β tubulin, b tubulin, Internal Transcribed Spacer, TP368-456, Aspergillus species, Restriction Fragment Length Polymorphism, restriction fragment length polymorphism, Food processing and manufacture, restriction enzymes
aspergillus species, internal transcribed spacer, β tubulin, b tubulin, Internal Transcribed Spacer, TP368-456, Aspergillus species, Restriction Fragment Length Polymorphism, restriction fragment length polymorphism, Food processing and manufacture, restriction enzymes
selected citations These citations are derived from selected sources.This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).0 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Average influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Average impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Average
Citations provided by BIP!Popularity provided by BIP!