Microplastics (MP) have spread to every corner of the globe, reaching remote areas like Antarctica. Recent studies detected MP in marine environments, including biota. Benthic organisms suffer negative effects upon MP ingestion, leading to impacts on their populations. To address the current knowledge gap on how Antarctic benthic invertebrates interact with MP, we conducted an experiment exposing a bivalve (Aequiyoldia eightsii) and two ascidians (Cnemidocarpa verrucosa and Molgula pedunculata) to polyethylene microbeads (mb). Specimens of each species were exposed for 48 h to two different concentrations of microbeads, a low dose (100 mb/l) and a high dose (1000 mb/l), with the same proportion of four different microbead size fractions (Fine (10-20 μm), Small (45-53 μm), Medium (106-125 μm), and Large (850-1000 μm)). After exposure, all three species had ingested microbeads. Significant differences between doses were observed in A. eightsii and C. verrucosa but not in M. pedunculata. Both ascidians ingested microbeads of all size fractions, whereas the bivalve did not ingest the largest microbeads. No significant differences were found between species in the number nor sizes of microbeads ingested. Minor variations between taxa may be attributed to the specific biology and anatomy of each species. Our study highlights the need for a deeper understanding of Antarctic benthic ecosystems, suggesting that the interaction with MP is species-specific. We believe that this study provides a baseline for assessing MP pollution in Antarctic benthic invertebrates and will help to inform policy-makers in protecting and preserving Antarctic marine ecosystems from MP pollution.