Objective: The purpose of our research was to study comparative cephalometric analysis between left and right sides of head and evaluate the skeletal facial symmetry in patients with class 1 malocclusion by 3D Cone-beam computed tomography (CBCT). Materials and Methods: Pre-treatment by 3D Cone-beam computed tomography radiographs for 20 patients of two age groups with class 1 malocclusion was undergone for 11 angular and 4 liner measurements from right and left sides, anteroposterior, vertical and frontal view by hand except the hard landmarks determination. Chi-test was used to compare the result of both sides (P < 0.05). Results: No difference was found between left and right analysis for linear measurements as for the angular cephalometric measurements. Conclusion: Measurements of skeletal analysis by 3D CBCT cephalograms from right side were found to be similar to left side. So, we can use the left analysis for orthodontics diagnosis as right side. Also, the both sides analysis can be used for symmetric analysis reasons.