Prenatal exposure to ethanol: A specific effect on the H19 gene in sperm
Copyright policy )Prenatal exposure to ethanol: A specific effect on the H19 gene in sperm
Alcohol exposure during pregnancy induces a range of disorders in the offspring. Methylation changes in imprinted genes may play a role in the teratogenic effects of alcohol. We evaluated the possible effects of alcohol administration in pregnant mice on the methylation pattern of 5 imprinted genes (H19, Gtl2, Peg1, Snrpn and Peg3) in somatic and sperm cell DNAs of the male offspring. The effects observed were a 3% (p < 0.005) decrease in the number of methylated CpGs of H19 in the F1 offspring sperm, a 4% (p < 0.005) decrease in the number of methylated CpGs of H19 in the F2 offspring brain and a 26% (p < 0.05) decrease in the mean sperm concentration. CpGs 1 and 2 of the H19 CTCF-binding site 2 exhibited significant methylation percentage losses. H19 CTCF-binding sites are important for the regulation of Igf2 gene expression. The hypomethylation of H19 may contribute to the decreased spermatogenesis in the offspring.
- Swiss Centre for Applied Human Toxicology Switzerland
- Geneva College United States
- University of Geneva Switzerland
- University Hospital of Geneva Switzerland
576.5, Spermatogenesis/drug effects/genetics, Male, RNA, Untranslated, Alcohol Drinking, RNA, Untranslated/genetics, CpG Islands/drug effects, Gestational Age, Brain/drug effects/embryology/metabolism, 618, Alcohol Drinking/adverse effects, Genomic Imprinting, Mice, Pregnancy, Ethanol/toxicity, Animals, Genomic Imprinting/drug effects, Spermatogenesis, Gene Expression Regulation, Developmental/drug effects, Ethanol, Sperm Count, Brain, Gene Expression Regulation, Developmental, DNA Methylation, DNA Methylation/drug effects, Spermatozoa/drug effects/metabolism, Spermatozoa, Maternal Exposure, Prenatal Exposure Delayed Effects, CpG Islands, Female, RNA, Long Noncoding, ddc: ddc:576.5, ddc: ddc:618
576.5, Spermatogenesis/drug effects/genetics, Male, RNA, Untranslated, Alcohol Drinking, RNA, Untranslated/genetics, CpG Islands/drug effects, Gestational Age, Brain/drug effects/embryology/metabolism, 618, Alcohol Drinking/adverse effects, Genomic Imprinting, Mice, Pregnancy, Ethanol/toxicity, Animals, Genomic Imprinting/drug effects, Spermatogenesis, Gene Expression Regulation, Developmental/drug effects, Ethanol, Sperm Count, Brain, Gene Expression Regulation, Developmental, DNA Methylation, DNA Methylation/drug effects, Spermatozoa/drug effects/metabolism, Spermatozoa, Maternal Exposure, Prenatal Exposure Delayed Effects, CpG Islands, Female, RNA, Long Noncoding, ddc: ddc:576.5, ddc: ddc:618
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