Objective of the work is to carry out comparative assessment of the pancreatic hydrolysates of protein-containing products, both phytogenous and zoogenous, as nutrient base for microbiological media. Gelatine, soy, soy concentrate, maize gluten, fish meal, common kilka, and bovine blood have been used as a feedstock. Protein stuff hydrolysis, hydrolysate purification, and validation of physical-chemical properties were performed in accordance with conventional techniques. Testing of peptone biological parameters has been carried out on the model of nutrient agar using Shigella flexneri 1a 8516, Shigella sonnei “S form”, Pseudomonas aeruginosa 27/99, Serratia plymuthica 1 test strains. Identified have been physical-chemical parameters of the hydrolysates under study. Detected are the variations in quantity, diameter and frequency of dissociation among the colonies of Shigella flexneri 1a 8516, Shigella sonnei “S form”, chromogenesis in Pseudomonas aeruginosa 27/99, Serratia plymuthica 1, cultivated on agar media with hydrolysates under study. Obtained are the comparative data on physical-chemical and biological parameters of all experimental hydrolysates, which offers an opportunity to differentiate their choice when adding them into bacteriological nutrient media.