Abstract Objective. Respiratory rate (RR) is an important vital sign but is often neglected. Multiple technologies exist for RR monitoring but are either expensive or impractical. Tri-axial accelerometry represents a minimally intrusive solution for continuous RR monitoring, however, the method has not been validated in a wide RR range. Therefore, the aim of this study was to investigate the agreement between RR estimation from a tri-axial accelerometer and a reference method in a wide RR range. Approach. Twenty-five healthy participants were recruited. For accelerometer RR estimation, the accelerometer was placed on the abdomen for optimal breathing movement detection. The acquired accelerometry data were processed using a lowpass filter, principal component analysis (PCA), and autocorrelation. The subjects were instructed to breathe at slow, normal, and fast paces in segments of 60 s. A flow meter was used as reference. Furthermore, the PCA-autocorrelation method was compared with a similar single axis method. Main results. The PCA-autocorrelation method resulted in a bias of 0.0 breaths per minute (bpm) and limits of agreement (LOA) = [−1.9; 1.9 bpm] compared to the reference. Overall, 99% of the RRs estimated by the PCA-autocorrelation method were within ±2 bpm of the reference. A Pearson correlation indicated a very strong correlation with r = 0.99 ( p < 0.001). The single axis method resulted in a bias of 3.7 bpm, LOA = [−14.9; 22.3 bpm], and r = 0.44 ( p < 0.001). Significance. The results indicate a strong agreement between the PCA-autocorrelation method and the reference. Furthermore, the PCA-autocorrelation method outperformed the single axis method.