The paper provides an overview of the tour guide activities in one of the oldest gardens in Russia - Peter the Great Botanical garden of Komarov Botanical Institute of Russian Academy of Sciences. It is the first time the authors have summarized the experience of working with visitors of the Peter the Great Botanical garden from the end of the 19th century to the present. Much attention is paid to the history of the excursion business in Russia and in the Botanical garden. The incentive to work was the growing interest in Botanical gardens and various forms of the excursion activity that now exist in the Botanical garden of Saint Petersburg. Modern science about excursions broadly describes general basics of conducting excursions, but practical techniques are not presented enough. Therefore, the task of methodological developments for the guides of the Botanical garden becomes relevant. Based on historical experience, the authors describe the rules for preparing for excursions in the Botanical garden, aspects when choosing information for excursions, special training methods, and general recommendations for excursions organization. Specific knowledge about the content of excursions in the Botanical garden will help specialists effectively and competently organize activities for environmental and biological education of both adults and young visitors.