In the flora of the republic, 41 Alchemilla species have been registered. Of the previously noted species, the presence of A. devestiens, A. glaucescens, and A. heptagona in the region is not confirmed by herbarium collections. The species A. atrifolia, previously listed for the republic but included in the most recent synopses only as a synonym of A. lindbergiana, is considered by us as a separate species. Previous works of co-authors have supplemented the Alchemilla list of the Mordovia flora with such species as Alchemilla argutiserrata, A. barbulata, A. cheirochlora, A. homoeophylla, A. kemlensis, A. longipes, A. mininzonii, A. psiloneura, A. pustynensis, A. schmakovii, A. sibirica, A. tichomirovii, A. vorotnikovii, and A. zimoenkensis. Here, two species are recorded for the first time for the republic – A. hians and A. prasina. The presence of A. litwinowii in Mordovia, which was previously noted exclusively on the basis of collections attributed subsequently to the newly described species A. tichomirovii, has also been confirmed. An original key, comprising all species of the genus registered in the middle part of the Volga River basin, has been compiled.