Comparative study of chikungunya Virus-Like Particles and Pseudotyped-Particles used for serological detection of specific immunoglobulin M

descriptionPublicationkeyboard_double_arrow_right Article 01 Mar 2019 English Publisher:Elsevier BVJournal:Virology, volume 529, pages 195-204 (issn: 0042-6822,

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Authors: Theillet, Gérald; Martinez, Jérôme; Steinbrugger, Christophe; Lavillette, Dimitri; Coutard, Bruno; Papageorgiou, Nicolas; Dalbon, Pascal; +2 Authors

doi: 10.1016/j.virol.2019.01.027

pmid: 30721816

Comparative study of chikungunya Virus-Like Particles and Pseudotyped-Particles used for serological detection of specific immunoglobulin M

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Abstract

The incidence of chikungunya virus (CHIKV) infection has increased dramatically in recent decades. Effective diagnostic methods must be available to optimize patient management. IgM-capture Enzyme-Linked Immunosorbent Assay (MAC-ELISA) is routinely used for the detection of specific CHIKV IgM. This method requires inactivated CHIKV viral lysate (VL). The use of viral bioparticles such as Virus-Like Particles (VLPs) and Pseudotyped-Particles (PPs) could represent an alternative to VL. Bioparticles performances were established by MAC-ELISA; physico-chemical characterizations were performed by field-flow fractionation (HF5) and confirmed by electron microscopy. Non-purified PPs give a detection signal higher than for VL. Results suggested that the signal difference observed in MAC-ELISA was probably due to the intrinsic antigenic properties of particles. The use of CHIKV bioparticles such as VLPs and PPs represents an attractive alternative to VL. Compared to VL and VLPs, non-purified PPs have proven to be more powerful antigens for specific IgM capture.

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Keywords

[SDV.BIO]Life Sciences [q-bio]/Biotechnology, Pseudotyped virus, 610, Viral/immunology*, Enzyme-Linked Immunosorbent Assay, MESH: Chikungunya virus/physiology*, Field-flow fractionation, Antibodies, Viral, Sensitivity and Specificity, [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases, Antibody Specificity, Humans, MESH: Serologic Tests/methods*, Serologic Tests, MESH: Antibody Specificity, Arbovirus, [SDV.MP.VIR] Life Sciences [q-bio]/Microbiology and Parasitology/Virology, [SDV.MHEP.ME] Life Sciences [q-bio]/Human health and pathology/Emerging diseases, [SDV.MHEP.ME]Life Sciences [q-bio]/Human health and pathology/Emerging diseases, MESH: Humans, MESH: Antibodies, MESH: Chikungunya Fever/diagnosis*, MESH: Enzyme-Linked Immunosorbent Assay, Virus-like particles., Virus-like particles, MESH: Sensitivity and Specificity, [SDV.BIO] Life Sciences [q-bio]/Biotechnology, MESH: Immunoglobulin M/immunology*, Immunoglobulin M, [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology, [SDV.MHEP.MI] Life Sciences [q-bio]/Human health and pathology/Infectious diseases, Chikungunya Fever, Chikungunya, Chikungunya virus

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