Therefore the objective of the present study was to characterize genetically wild and cultivated populations of P. magdalenae, using molecular markers type RAMs in the area of the lower San Jorge, in the Department of Sucre. For this, DNA was extracted and PCR amplified with seven primers RAMs, in a sample of 80 wild specimens from four sites and 40 cultured specimens from two companies the. All primers RAMs were polymorphic with values ranging from 93,33% in the CA primer to 99,17% in AG, this primer had the highest He value (0,469 ± 0,005), while the CCA primer had the lowest value (0,321 ± 0,010). In the wild populations of P. magdalenae the SBA site was the most diverse (He: 0,194 ± 0,028) and the CAI site was the least diverse (0,150 ± 0,029), with an He average of 0,167 ± 0,019, that was lower than the average often cultivated population (He: 0,194 ± 0,002). The analysis of the population structure reflects greater variation within that between the populations with moderate and high FST values, indicating low gene flow between sites and between wild and cultivated populations, that is evidenced in dendrograms. It is concluded that this genetic structure can be due to natural or artificial physiographic barriers and that the greater diversity in the cultivated population can be due to an adequate reproductive management or endogamy effects in the wild population.