In the areas of serpentines live wild species whose propagation for traditional roads is not possible because they present problems in the germination of the seeds under nursery conditions. The main objective of this work was to achieve the establishment in vitro of Erythroxilum echynodendron, in order to increase the number of available postures for the restoration in the administrative unit “El Recreo” located in the protected area Cubanacán. For the in vitro establishment of the seeds the effect of a Sodium Hypochlorite solution (2.0% NaOCl) during different times of disinfection (15, 20, 25 min.) was studied. Whole seeds and seeds which the embryonic sack with embryo inside (the pericarp and the endosperm were moved away) were sowed in the culture medium composed by 50% of the MS salts and 2.0% of sucrose. The bigger percentage of seeds free of microbial pollutants (60%) was achieved when the treatment with 2.0% of NaOCL during 25 minutes was used. The biggest percentage of germinated seeds (80.7%) was achieved when the embryonic sack with the embryo inside was sowed in the culture medium. Key words: Tissue culture, threatened species, biodiversity conservation, cuabal, endemic species