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Study of colpo-cytological at the capybaras (Hydrochoerus hydrochaeris) Caracterização da colpocitologia em capivaras ("Hydrochoerus hydrochaeris")

Authors: Cláudia Diniz Pinto Coelho; Maytê Koch Balarini; Bruno Edésio dos Santos Melo; Danielle Barbosa Morais; Mariana Machado Neves; Marcos Vinícius Rodrigues; Larissa Pires Barbosa; +1 Authors

Study of colpo-cytological at the capybaras (Hydrochoerus hydrochaeris) Caracterização da colpocitologia em capivaras ("Hydrochoerus hydrochaeris")

Abstract

Capybara (Hydrochoerus hydrochaeris) is one of the species which has interested farmers due to its performance potential, as a result of the quality of its skin and meat. Based on commercial interests, many studies have been done to understand the reproductive physiology and to answer questions that could help to improve the productive status of these animals. This study was performed to describe the cell types from the vaginal epithelium of capybaras and to compare the efficiency of three dyes on cell staining. The experiment was carried out at Centro de Biodiversidade of USIPA, in Ipatinga/MG. Cells were obtained by the swab technique from five adult animals and stained with Giemsa, Methylene Blue and Shorr. Samples were collected every three and five days, during 30 days. Four types of cells were observed, parabasal cells, intermediate cells and superficial cells (with or without nucleus), besides bacteria and leukocytes. The three dyes were able to stain efficiently on colpocitology, however, Giemsa and Metileno Blue were more practical than Shorr. Colpocytology was shown to be an applicable procedure for this animal species.A capivara (Hydrochoerus hydrochaeris) é uma das espécies que tem despertado o interesse de produtores rurais pelo potencial zootécnico, advindo da qualidade do seu couro e da sua carne. Com base no interesse comercial, muitas pesquisas têm sido desenvolvidas para se entender a fisiologia reprodutiva e responder questões que possam ajudar no aumento da produção desses animais em cativeiro. Este estudo foi conduzido com o objetivo de descrever as células do epitélio vaginal de capivaras e comparar a eficiência de três corantes na coloração das mesmas. O experimento foi realizado no Centro de Biodiversidade da USIPA em Ipatinga / MG. As células foram obtidas pela técnica de swab vaginal de cinco fêmeas adultas cativas e coradas com: Giemsa, Azul de Metileno e Shorr. Foram realizadas coletas com intervalos de 3 e 5 dias, durante um período de 30 dias. Foram encontrados quatro tipos celulares, as células parabasais, células intermediárias e células superficiais (nucleadas e anucleadas), além de células bacterianas e leucócitos. Os três corantes agiram de forma eficiente nas células da colpocitologia, contudo os corantes Giemsa e Azul de Metileno apresentaram maior praticidade, em relação ao corante Shorr. A colpocitologia mostrou-se uma técnica aplicável nessa espécie.

Keywords

Aquaculture. Fisheries. Angling, SH1-691, SF1-1100, Animal culture

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
0
Average
Average
Average
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