The infertility problem named Repeat Breeder Syndrome is an important issue in cattle breeding. Beside maintenance and feed techniques, some genetic disorders such as Robertsonian Translocation and factor XI deficiency (FXID) are thought to be involved some problems in Holstein cattle. In this study, it was aimed to investigate the presence of Robertsonian Translocation and FXID in repeat breeder Holstein cows which grown a farm that located in the province of Kayseri. In the study 62 female Holstein cattle with Repeat Breeder Syndrome were examined. Chromosomal examination results indicated that 58 of 62 cows had a normal karyotype (2n= 60 diploid) and four had different of Robertsonian translocation [Rob (1, 21), rob (23, 26), Rob (24, 26), Rob (26, 29)] profiles. None of the 62 cows was displayed the band of mutation which is the cause of FXID. As a result of the study it was determined molecular and cytogenetical screening of cows with a fertility problem could contribute to understand the reasons of repeat breeder syndrome and could be useful for the breeders to select the female breeding candidates and finding the solution for this problem.