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handle: 10261/332716
Horizon 2020 (H2020), grant/award no. 817806: Sustainable management of mesopelagic resources; Ministerio de Ciencia e Innovación (MICINN), grant/award no. CTM2012-39587-C04: Migrants and Active Flux In the Atlantic Ocean; Ministerio de Ciencia e Innovación (MICINN), grant/award no. CTM2015-69392-C3: Constraining organic carbon fluxes in an eastern boundary upwelling ecosystem (NW Africa): the role of non-sinking carbon in the context of the biological pump; Ministerio de Ciencia e Innovación (MICINN), grant/award no. CTM2017-83362-R: INTERES: Papel de las interacciones fitoplancton-bacterias en la respuesta del plancton microbiano a la entrada de nutrientes alóctonos; Ministerio de Ciencia e Innovación (MICINN), grant/award no. PID2019-109084RB-C21: Biogeochemical impact of mesoscale and sub-mesoscale processes along the life history of cyclonic and anticyclonic eddies: plankton variability and productivity
Samples were collected in the tropical and subtropical Atlantic during the MAFIA cruise (April 2015) on board the BIO Hespérides. Seawater samples were collected at 13 stations (from the Brazilian coast to the Canary Islands), from the surface down to 3500 m, using a General Oceanics oceanographic rosette equipped with 24 l PVC Niskin bottles. Abundance and cell characteristics (high nucleic acid content fraction, cell volume, viability) were based on measurements performed with a FACSCalibur (Becton-Dickinson) flow cytometer. Leucine incorporation rates were estimated with tritiated leucine (Kirchman et al. 1985) using centrifugation and filtration methods (Smith and Azam 1992). The aim of this dataset was to estimate the influence of surface productivity on the standing stock, characteristics and activity (as leucine incorporation) of prokaryotes across the water column
This dataset contains the results of the characterisation of the prokaryotic community by flow cytometry and tritiated leucine incorporation from the MAFIA cruise (Migrants and Active Flux In the Atlantic ocean)
With the institutional support of the ‘Severo Ochoa Centre of Excellence’ accreditation (CEX2019-000928-S)
No
Deep ocean, History, potential, High nucleic acid prokaryotes low nucleic acid prokaryotes ratio, Cell volume, Mesopelagic, Calculated after Norland (1993), Calculated, Biomass, Prokaryotes, cell volume, Bathypelagic, prokaryote, Sea surface chlorophyll a, http://aims.fao.org/aos/agrovoc/c_689, cytometry, Biogeochemical impact of mesoscale and sub mesoscale processes along the life history of cyclonic an, Conductivity-Temperature-Depth- profiler (CTD), Sea-Bird Scientific, SBE 911plus, Calculated after Norland 1993, Prokaryotes, biomass as carbon, Station label, Pressure, water, Constraining organic carbon fluxes in an eastern boundary upwelling ecosystem NW Africa the role of, Longitude of event, leucine incorporation, water, Migrants and Active Flux In the Atlantic Ocean MAFIA, High nucleic acid prokaryotes, bathypelagic, Calculated after Calvo Díaz and Morán 2006, Microbial community, http://aims.fao.org/aos/agrovoc/c_4292, Pressure, Date/Time of event, Temperature, water, potential, Specific 3H-leucine incorporation rate, Specific 3H leucine incorporation rate, biomass, viability, Event label, SCF, Fluorometer, Seapoint Sensors inc., SCF, Sea Bird Scientific, Calculated after Calvo-Díaz and Morán (2006), NADS, Cytometry, Salinity, Fluorometer, Seapoint, SCF, Conductivity-Temperature-Depth- profiler (CTD), Sea-Bird Scientific, SBE911 plus, Chlorophyll a, Fluorometer, CTD/Rosette, Latitude of event, mesopelagic zone, High nucleic acid prokaryotes/low nucleic acid prokaryotes ratio, mesopelagic, Prokaryotes, Migrants and Active Flux In the Atlantic Ocean (MAFIA), biomass as carbon, Atlantic Ocean, cell volume, Productivity, Hespérides, Tritiated leucine incorporation (Kirchman et al. 1985) with centrifugation and filtration methods (Smith and Azam 1992), Viable cell fraction, Seapoint, Temperature, Biogeochemical impact of mesoscale and sub-mesoscale processes along the life history of cyclonic and anticyclonic eddies: plankton variability and productivity (e-IMPACT), SBE911 plus, MAFIA, CTD Rosette, Conductivity Temperature Depth profiler CTD, Prokaryotic abundance, http://aims.fao.org/aos/agrovoc/c_926, deep ocean, Low nucleic acid prokaryotes, Viability, Prokaryote, leucine, microbial community, productivity, Leucine incorporation, http://aims.fao.org/aos/agrovoc/c_74c879b7, FACSCalibur flow cytometer Becton Dickinson, Campaign, Tritiated leucine incorporation Kirchman et al 1985 with centrifugation and filtration methods Smith, Humanities, FACSCalibur flow-cytometer (Becton Dickinson), Bulk 3H leucine incorporation rate, Constraining organic carbon fluxes in an eastern boundary upwelling ecosystem (NW Africa): the role of non-sinking carbon in the context of the biological pump (FLUXES), DEPTH, water, Date Time of event, Sustainable Management of Mesopelagic Resources SUMMER, Sustainable Management of Mesopelagic Resources (SUMMER), Sample ID, DEPTH, Atlantic, Bulk 3H-leucine incorporation rate, prokaryotic abundance
Deep ocean, History, potential, High nucleic acid prokaryotes low nucleic acid prokaryotes ratio, Cell volume, Mesopelagic, Calculated after Norland (1993), Calculated, Biomass, Prokaryotes, cell volume, Bathypelagic, prokaryote, Sea surface chlorophyll a, http://aims.fao.org/aos/agrovoc/c_689, cytometry, Biogeochemical impact of mesoscale and sub mesoscale processes along the life history of cyclonic an, Conductivity-Temperature-Depth- profiler (CTD), Sea-Bird Scientific, SBE 911plus, Calculated after Norland 1993, Prokaryotes, biomass as carbon, Station label, Pressure, water, Constraining organic carbon fluxes in an eastern boundary upwelling ecosystem NW Africa the role of, Longitude of event, leucine incorporation, water, Migrants and Active Flux In the Atlantic Ocean MAFIA, High nucleic acid prokaryotes, bathypelagic, Calculated after Calvo Díaz and Morán 2006, Microbial community, http://aims.fao.org/aos/agrovoc/c_4292, Pressure, Date/Time of event, Temperature, water, potential, Specific 3H-leucine incorporation rate, Specific 3H leucine incorporation rate, biomass, viability, Event label, SCF, Fluorometer, Seapoint Sensors inc., SCF, Sea Bird Scientific, Calculated after Calvo-Díaz and Morán (2006), NADS, Cytometry, Salinity, Fluorometer, Seapoint, SCF, Conductivity-Temperature-Depth- profiler (CTD), Sea-Bird Scientific, SBE911 plus, Chlorophyll a, Fluorometer, CTD/Rosette, Latitude of event, mesopelagic zone, High nucleic acid prokaryotes/low nucleic acid prokaryotes ratio, mesopelagic, Prokaryotes, Migrants and Active Flux In the Atlantic Ocean (MAFIA), biomass as carbon, Atlantic Ocean, cell volume, Productivity, Hespérides, Tritiated leucine incorporation (Kirchman et al. 1985) with centrifugation and filtration methods (Smith and Azam 1992), Viable cell fraction, Seapoint, Temperature, Biogeochemical impact of mesoscale and sub-mesoscale processes along the life history of cyclonic and anticyclonic eddies: plankton variability and productivity (e-IMPACT), SBE911 plus, MAFIA, CTD Rosette, Conductivity Temperature Depth profiler CTD, Prokaryotic abundance, http://aims.fao.org/aos/agrovoc/c_926, deep ocean, Low nucleic acid prokaryotes, Viability, Prokaryote, leucine, microbial community, productivity, Leucine incorporation, http://aims.fao.org/aos/agrovoc/c_74c879b7, FACSCalibur flow cytometer Becton Dickinson, Campaign, Tritiated leucine incorporation Kirchman et al 1985 with centrifugation and filtration methods Smith, Humanities, FACSCalibur flow-cytometer (Becton Dickinson), Bulk 3H leucine incorporation rate, Constraining organic carbon fluxes in an eastern boundary upwelling ecosystem (NW Africa): the role of non-sinking carbon in the context of the biological pump (FLUXES), DEPTH, water, Date Time of event, Sustainable Management of Mesopelagic Resources SUMMER, Sustainable Management of Mesopelagic Resources (SUMMER), Sample ID, DEPTH, Atlantic, Bulk 3H-leucine incorporation rate, prokaryotic abundance
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