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FactorForge: constraint-based CDS design engine for plant expression workflows

integration_instructionsResearch softwarekeyboard_double_arrow_right Software Under curationPublisher:Zenodo
Authors: Kim, Mun-Kyu;

doi: 10.5281/zenodo.20538154

FactorForge: constraint-based CDS design engine for plant expression workflows

Abstract

FactorForge is an open-source constraint-based coding sequence (CDS) design engine for plant expression workflows, with initial support for Nicotiana benthamiana (agroinfiltration) and Tobacco BY-2 suspension culture cells (--host by2). It maximizes the Codon Adaptation Index (CAI) under simultaneous GC content, PolyA signal, rare codon, and restriction site constraints using a dynamic programming feasibility engine, producing MoClo/Golden Gate-ready constructs with full provenance tracking.

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