The objective of this research work is to investigate some biochemical parameters in the liver and kidney of alloxan-induced diabetic rats treated with Senecio biafrae leaf aqueous extract. Alloxan-induced diabetic rats (150mg/kg) were administered orally with Senecio biafrae leaf aqueous extract (200mg/kg and 400mg/kg) for fifteen days after which some biochemical indices in the serum, liver and kidney were measured and compared with the control. The results shows that serum and tissues alkaline phosphatase (ALP), alanine aminotransferase (ALT), and aspartate aminotransferase (AST), serum urea, direct bilirubin and creatinine of untreated diabetic group were significantly higher (p<0.05) when compared with the normal control group and extract treated group, while significant reduction were observed in serum total protein, albumin and globulin of diabetic untreated group when compared to both extract treated and normal control groups. It can be concluded that administration of aqueous leaf extract of Senecio biafrae to diabetic rats did not have any adverse effect on the liver and kidney functions indices in rats, instead it ameliorate the adverse effects of diabetes complications.