In this paper, a comparison of Microstrip patch antenna parameters returns loss, VSWR and directivity between using HFSS Software and Artificial Neural Network. In this antenna achieved four resonant frequency 3.5 GHz, 6 GHz, 6.9 GHz and 8.9GHz using substrate material Fr-4 Glass epoxy substrate with using co-axial feeding technique. An ANN has been developed for the calculation of return loss, VSWR and directivity of resonant microstrip antenna using MATLAB and compare with HFSS’s antenna parameters. The resonated frequencies lies in S, C and X bands. Results of the ANN network are compared with the data obtained from HFSS software and concluded that ANN results are in good agreement as compared to HFSS.