Turnover affects the cost of operations and drains the organization of inherent tacit knowledge. Employee voluntary turnover is very costly for organizations, particularly for the health care sector, where the costs of their labour force are expensive. Employees' voluntary turnover has substantial negative impacts on the organizations especially costs, potential loss of valuable knowledge, skills and organizational knowledge. That is why it becomes a critical issue to take address. Studies have been carried out on the reasons which lead to this tendency; however the ability to explain and predict it remains restricted. So it becomes necessary for human resource managers to explore the reasons for job dissatisfaction of employees in health care sector. The objective of this study is to examine the relationship between Job satisfaction and turnover of nurses in health care sector belongs to Ernakulum District of Kerala. Data was collected from 100 nurses, using self-reporting questionnaires. Findings were made with the help of SPSS package. The study revealed that there is negative correlation between Job Satisfaction and employee turnover. The empirical results show that job satisfaction has a negative effect on employee voluntary turnover among the subjects of this study. The finding in this research could be adopted by organizations and managements who should develop a way to prevent voluntary turnover among their employees and develop a route to increase their employees ‘job satisfaction’ and from that point to reduce voluntary turnover and retain this expensive human resource in organisations particularly in health care sector.