Objective: -To investigate the anti diabetic and anti Hyperlipidemic activity of methanol extract of Barleria longiflora in male Wistar rats. Material & method: - In this model of Hyperlipidemia, 30 adult male wistar rats (150-200gms) were evenly divided into 5 groups in both groups. Group-1 and Group-2 served as untreated and model controls respectively, while Group-3, 4 and 5 were the treatments groups which were simultaneously treated with standard, 200 and 400 mg/kg extract respectively along with High Fat Diet and Triton x 100. On last day, blood samples for biochemical parameters, were obtained under inhaled diether anaesthesia. In the model of anti diabetic animals were evenly divided into 5 groups Group-1 and Group-2 served as untreated and model controls respectively, while Group-3, 4 and 5 were the treatments groups which were simultaneously treated with standard, 200 and 400 mg/kg extract respectively after glucose loading. Results: - HFD and Triton x 100 treatment caused Hyperlipidemia as evidenced by marked elevation in Cholesterol, Triglycerides, LDL, VLDL and decrease in HDL levels. Co-administration of extract with HFD and Triton x 100 decreased rise Cholesterol, Triglycerides, LDL, VLDL and increase in HDL levels. Glucose loading caused hyperglycemia by elevation of glucose which was significantly reduced by treatment with standard and extract. Conclusion: It was observed that the methanol extract of Barleria longiflora conferred anti diabetic and Anti- Hyperlipidemia activity by biochemical observation against HFD and Triton-x-100 induced Hyperlipidemia in rats. In the near future could constitute a lead to discovery of a novel drug for treatment of drug induced Hyperlipidemia and diabetes.