This Dataset includes matlab variables containing all raw and preprocessed data 1) fiber photometry experiments (GCaMP and GFP) 2) miniscope experiments 3) optogenetics experiments 4) DLC video analysis for photometry recording, optogenetic inhibition ArchT, optogenetic activation ChR2, optogenetic activation ChR2 + MK801, control experiments for optogenetic inhibition and activation 5) Source Data Files for all main and supplementary Figures collected for the paper "Striatum-projecting prefrontal cortex neurons support working memory maintenance" Maria Wilhelm1,2,6, Yaroslav Sych1,7, Aleksejs Fomins1,2, José Luis Alatorre Warren1,8, Christopher Lewis1, Laia Serratosa Capdevila1, Roman Boehringer3, Elizabeth A. Amadei3, Benjamin Grewe2,3,4, Eoin C. O'Connor5, Benjamin J. Hall5,9, Fritjof Helmchen1,2,4* 1Brain Research Institute, University of Zurich, 8057 Zurich, Switzerland. 2Neuroscience Center Zurich, University of Zurich and ETH Zurich, 8057 Zurich, Switzerland. 3Institute of Neuroinformatics, University of Zurich and ETH Zurich, 8057 Zurich, Switzerland. 4University Research Priority Program (URPP) Adaptive Brain Circuits in Development and Learning (AdaBD), University of Zurich, Zurich, Switzerland 5Neuroscience & Rare Diseases, Roche Pharma Research and Early Development, Roche Innovation Center Basel, F. Hoffmann-La Roche Ltd, Basel, Switzerland. 6Present address: Institute for Neuroscience, ETH Zurich, 8057 Zurich, Switzerland. 7Present address: Institute of Cellular and Integrative Neuroscience, CNRS, University of Strasbourg, Strasbourg, France. 8Present address: Center for Lifespan Changes in Brain and Cognition, University of Oslo, Oslo 0317, Norway. 9Present address: Circuit Biology Department, H. Lundbeck A/S, Valby, Denmark. These authors contributed equally: Maria Wilhelm, Yaroslav Sych *email: helmchen@hifo.uzh.ch