Abstract. The Tibetan Plateau (TP; approximately 27.5–37.5° N, 75.5–105.5° E) is the highest and largest plateau on Earth with a mean elevation of over 4 km. This special geography causes strong surface solar ultraviolet radiation (UV), with potential risks to human and ecosystem health, which is mainly controlled by the local stratospheric ozone concentration. The El Niño–Southern Oscillation (ENSO), the dominant mode of interannual variability on Earth, is characterised by the tropical Pacific Ocean sea surface temperature anomalies (SSTAs) and sea level pressure change for the warm-phase El Niño and cold-phase La Niña events. Although some studies have suggested the existence of positive correlation between ENSO and the total column ozone (TCO) over the TP, the mechanism underlying this effect is not fully understood. Here we use the Copernicus Climate Change Service (C3S) merged satellite dataset, the Stratospheric Water and OzOne Satellite Homogenized (SWOOSH) dataset and the TOMCAT three-dimensional (3D) offline chemical transport model forced by ERA5 meteorological reanalyses from the European Centre for Medium-Range Weather Forecasts (ECMWF) over the period 1979–2021 to investigate the influence of ENSO on the TCO over the TP. We find that the El Niño (La Niña) events favour positive (negative) TCO anomalies over the TP from wintertime of its mature phase to springtime of its decaying phase. Through studying the ozone profile, we attribute the positive (negative) TCO anomalies mainly to the increased (decreased) ozone at the 200–70 hPa levels, i.e. in the upper troposphere and lower stratosphere (UTLS) regions. Our results suggest that the El Niño events impact the TP TCO via the following potential processes: (1) a negative upper-level geopotential height anomaly associated with El Niño is responsible for a decrease in air column thickness; (2) the thickness decrease modulates reduced tropospheric temperature and thus favours a decrease in the tropopause height (TH); and (3) such a TH decrease tends to induce a change in the relative amounts of ozone-poor tropospheric and ozone-rich stratospheric air in the profile, which increases the partial column ozone in the UTLS and hence corresponds to the TP TCO increase. The La Niña events affect TP TCO in a manner resembling the El Niño events, except with anomalies of opposite sign. This work provides a systematic understanding of the influence of ENSO on ozone over the TP, which has implications for the interannual variability of ozone.