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Integrative structure determination of PTBP1-viral IRES complex in solution

Authors: Dorn, Georg; Gmeiner, Christoph; de Vries, Tebbe; Dedic, Emil; Novakovic, Mihajlo; Damberger, Fred F.; Maris, Christoph; +11 Authors

Integrative structure determination of PTBP1-viral IRES complex in solution

Abstract

Ensemble structure model of the RNA-binding protein PTBP1 in complex with the internal ribosome entry site (IRES) of encephalomyocarditis virus (EMCV) RNA and data underlying these models. Main ensemble based on all restraints (corresponding to Figure 2 in the associated paper) Ensemble obtained with only DEER distance distribution restraints corresponding to Figure S7(A) in the Supplementary Material of the associated paper Validation ensemble obtained with all restraints after removing the conformers of the main ensemble from the raw ensemble corresponding to Figure S7(B) inthe Supplementary Material of the associated paper Ensemble obtianed with all restraints by fitting populations with a non-negative linear least squares (NNLLSQ) approach corresponding to Figure S8(A) in ths Supplementary Material of the associated paper Primary DEER-EPR data underlying site-to-site distance distributions for 35 spin-label pairs and corresponding distanace distributions Small-angle neutron scattering (SANS) curves a two detector distances with corresponding resolution files and a small-angle x-ray scattering (SAXS) curve Restraint file for the ensemble fit with MMMx software, specifying the mean distances and standrad deviations of distance distributions that were also used for specifying lower and upper distance bounds in CYANA generation of the raw ensemble Source data for the figures in the associated paper Source data for the tables in the associated paper All ensembles are ZIP files containing single PDB files for all conformers and an ensemble specification that reports populations for all conformers.

additional funding: Schweizerischer Nationalfonds zur Fórderung der Wissenschaften 31003A-170130 Schweizerischer Nationalfonds zur Förderung der Wissenschaften Sinergia CRSII5-170976 ETH Research Grant 0-2066-14

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Keywords

ensemble modelling, SANS, RNA-binding protein, biophysics, structural biology, EPR, SAXS, virus, NMR

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
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