Numerous research applications require that the order of nucleic acid residues in biological samples be determined. Numerous scientists have worked hard over the past 50 years to develop the methods and tools necessary to accomplish this feat of sequencing DNA and RNA molecules. The sequencing of small oligonucleotides has given way to sequencing millions of bases and the struggle to deduce the coding sequence of a single gene has given way to whole genome sequencing that is quick and widely accessible. The sequence of the nucleotides in a DNA molecule is determined via sequencing technology. Each creature has a certain order that determines who we are, how we act, and how we respond to a changing environment. Next Generation Sequencing and Sanger are the two primary methods.