Machine tools with a hybrid kinematic structure represent an unconventional type of machine tools whose characteristics depend on the kinematic configuration. Therefore, it is necessary to perform a separate accuracy analysis for each machine configuration. The analysis of the characteristic values obtained by examining the accuracy of positioning is the starting point for further improvement and calibration of this type of machine. In the case of conventional machine tools with a serial kinematic structure, appropriate standards and recommendations are used to evaluate the state of the machine through testing the positioning accuracy. The paper describes the preliminary measurement of the condition of the machine tool with the hybrid kinematic structure "O-X glide" according to the ISO 230-2 standard, carried out in order to adjust the mechanical subassemblies of the machine, which precedes the process of calibrating the machine's control system.