Spectral decomposition coefficients, spatiotemporal gene expression pattern identification and multidimensional scaling coordinates of spatiotemporal transcriptomics data and image analysis results used to create Figure 2 in the scientific article "Simultaneous spatiotemporal transcriptomics and microscopy of Bacillus subtilis swarm development reveal cooperation across generations" by the following authors: Hannah Jeckel*, Kazuki Nosho*, Konstantin Neuhaus, Alasdair D. Hastewell, Dominic J. Skinner, Dibya Saha, Niklas Netter, Nicole Paczia, Jörn Dunkel, Knut Drescher. The symbol "*" indicates an equal contribution. This data consists of two excel sheets, one for the transcriptomics data and one for image analysis results (physical properties). Genes were ranked according to a spatiotemporal information score defined in the publication described above. For each gene, its name and ID (derived from locus tag) are given as identifiers. For information of reference genome used for mapping and convention on how gene names are chosen, see https://drescherlab.org/data/swarm-transcriptome/. Other columns in the sheet represent the spatiotemporal information score, assigned spatiotemporal pattern number, decomposition coefficient, multidimensional scaling coordinates and gene function. Physical properties are measured from short microscopy videos and defined in the publication mentioned above. This excel sheet contains property name, spatiotemporal information score, spectral decomposition coefficients and multidimensional scaling coordinates.