SRCD data of two protein constructs from human hepatocyte nuclear factor 1A (HNF-1A, UniProt ID: P20823). Protein construct "DBD" harbors the DNA-binding domain of HNF-1A (residues 83-279). Protein construct "DBD-TAD" harbors the DNA-binding domain and the transactivation domain of HNF-1A (residues 83-631), as well as an N-terminal His-tag with a short linker. DBD-TAD was measured as a TFE titration series (0% - 70% TFE). Data was collected on the AU-CD beamline, ASTRID2 synchrotron (Aarhus, Denmark). The compressed file contains a directory with separate folders for SRCD raw files (baseline and sample scans), processed subtracted data files, and information regarding methodology and sample details.

We acknowledge the use of the Core Facility for Biophysics, Structural Biology, and Screening (BiSS) at the University of Bergen (Bergen, Norway), which has received infrastructure funding from the Research Council of Norway (RCN) through NORCRYST (grant number 245828) and NOR–OPENSCREEN (grant number 245922). This work was funded with a PhD fellowship by the Medical Faculty, University of Bergen, UiB, Norway (to L. K.) and by project grants awarded by the UiB Meltzer foundation (to L. K.). We thank BioCat - The National graduate school in biocatalysis - for financial support connected to beamtime travel.