Calmodulin is an intermediate calcium binding receptor messenger protein expressed in every eukaryotic cells. This is a very important factor in the Arabidopsis thaliana. Characterization and expression analysis of CAM2 were conducted to obtain important information of its function and regulation control under several stress condition. The study included ePlant heat map representation data, GO and KEGG analysis pathway interpretation. ePlant data gives us the insights of various tissue specific and cellular localization through heat map data. GO analysis data gives us inputs about molecular function, biological processes and cellular components. KEGG pathway analysis data represents how CAM2 expresses over any stress condition, how it is related with various conserved signalling pathways. Overall, these findings give us the answer that how each and every, environmental factors are connected with each other and how these organisms act upon certain environmental factors. The one of the most important findings from this characterization and expression analysis were, CAM2 is very important to response to several biotic factors such as pathogen interaction. CAM2 is highly responsible for the growth of mature pollen of Arabidopsis thaliana.