The purpose of this study was to analyze the influence of knowledge, abilities and i(Ningrum, 2020) information technology on organizational performance in RSAU dr. Esnawan Space Halim Perdana Kusuma Jakarta, either simultaneously or partially. This study also wants to analyze which variables of knowledge, abilities and information technology have a dominant effect on organizational performance in RSAU dr. Esnawan Space Halim Perdana Kusuma Jakarta. The design of research is explanatory research. The population study was all members of the first officer in RSAU dr. Esnawan Space Halim Perdana Kusuma Jakarta, with 46 members. Considering that the population was not too large, a census study was used, namely all members of the first officer in RSAU dr. Esnawan Space Halim Perdana Kusuma Jakarta will be the research respondent. The analysis method uses multiple linear regression and hypothesis testing. The results have shown that the knowledge, abilities and information technology on the organizational performance of the first officer members in RSAU dr. Esnawan Space Halim Perdana Kusuma Jakarta, either simultaneously or partially. Furthermore, this study identifies that knowledge has a dominant effect on organizational performance in RSAU dr. Esnawan Space Halim Perdana Kusuma Jakarta.