Gene editing using CRISPR/Cas9 to induce new mutations in the SYP121 gene in Arabidopsis and barley

Sayed, Haitham; Lawrenson, Tom; Hinchliffe, Alison; Amtmann, Anna; Harwood, Wendy; Blatt, Michael

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Conference object . 2018

License: CC BY

Data sources: Datacite

ZENODO

Other literature type . 2018

License: CC BY

Data sources: ZENODO

Gene editing using CRISPR/Cas9 to induce new mutations in the SYP121 gene in Arabidopsis and barley

descriptionPublicationkeyboard_double_arrow_right Conference object , Other literature type 25 Jun 2018 English Publisher:Zenodo

Authors: Sayed, Haitham; Lawrenson, Tom; Hinchliffe, Alison; Amtmann, Anna; Harwood, Wendy; Blatt, Michael;

doi: 10.5281/zenodo.8315818

Gene editing using CRISPR/Cas9 to induce new mutations in the SYP121 gene in Arabidopsis and barley

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Abstract

We used the SYP121 gene as a target for CRISPR/Cas 9 based genome editing in both Arabidopsis and barley. We identified the barley SNARE with the FxRF motif in the first 15 residues of the protein in the target genotype Golden Promise. We then designed constructs that were expected to knock-out this part of the gene in Arabidopsis and in barley.

Keywords

CRISPR/Cas9, Barley, Arabidopsis, Off-target, SYP121 gene.

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