At significant confinement levels, the potent active stress disrupts the alignment of the flagella, leading to a turbulent state in the composite liquid crystal. Conversely, minimal confinement enables the dominant nematic alignment to counteract the active stress, inducing the phase separation of microtubules within a uniform flagella background. The confocal micrograph unveils uniform flagella distribution across the medium, while the microtubules segregate into a distinct lane-like structure within the channel's narrowest confinement.

[kinesin motor clusters] 120nM, [Microtubules] 1.33mg/ml, [ATP] 1.4mM, ATP regenerating system: PEP-PKLADH