Fig. 2. Phylogenetic analysis of SoBDH1, SoBDH2 and related ADHs. The tree was constructed using the neighbour-joining algorithm with Clustal O and visualized with Mega 7. Enzyme abbreviation, NCBI-accession number and species are following (enzymes used in this study marked in bold): LiBDH (LiBDH_0259) - borneol dehydrogenase from Levandula intermedia (AFV30207.1), AaBDH (AaBDH_0294) - borneol dehydrogenase from Artemisia annua (ANJ65952.1), ADH2 (AaADH_0265) - alcohol dehydrogenase from Artemisia annua (ADK56099.1), PsBDH (PsBDH_0261) - borneol dehydrogenase from Pseudomonas sp. TCU-HL1, SoBDH1 (SoBDH1_0259) - borneol dehydrogenase 1 from Salvia officinalis L., SoBDH2 (SoBDH2_0283) - borneol dehydrogenase 2 from Salvia officinalis L., PySDH_0270 - Secoisolariciresinol dehydrogenase from Prunus yedoensis var. nudiflora (PQQ15129.1), AaBDH2_0293 - borneol dehydrogenase from Artemisia annua (PWA65158.1), AaADH2_0265 - alcohol dehydrogenase from Artemisia annua (PWA54131.1), PpSCDH_0285 - putative short-chain dehydrogenase/reductase SDR from Pseudomonas putida (BAN13298.1). EgHYP_0318 - hypothetical protein MIMGU_mgv1a010226mg from Erythranthe guttata (EYU18089.1), DhSCDH_0290 - short chain alcohol dehydrogenase from Dorcoceras hygrometricum.

Published as part of Drienovská, Ivana, Kolanović, Dajana, Chánique, Andrea, Sieber, Volker, Hofer, Michael & Kourist, Robert, 2020, Molecular cloning and functional characterization of a two highly stereoselective borneol dehydrogenases from Salvia officinalis L, pp. 1-8 in Phytochemistry (112227) 172 on page 2, DOI: 10.1016/j.phytochem.2019.112227, http://zenodo.org/record/8294329