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Aluminium Induced Microscopic Changes in the Kidney

Authors: Shilpi Jain,; Satyam Khare,; Archana Sharma,; Virendra Budhiraja,; Rastogi, Rakhi;

Aluminium Induced Microscopic Changes in the Kidney

Abstract

{"references": ["1. Alfrey A.C: Aluminium. Advance in Clinical Chemistry, 1983;23:69-91. 2. Braunlich H, Fleck Ch, Kersten L, Stein G, Laske V, Muller A, Keil E: Renal effects of aluminum in uraemic rats and in rats with intact kidney function. Journal of Applied Toxicology, 1986; 6(1):55-59. 3. Braunlich H, Penzold K, Tiersch W, Fleck C, Winnefield K, Stein G: Pharmacokinetic pattern and renal effects of aluminium in rats with impaired liver or kidney function. Trace Element in Medicine, 1988; 5(1):38-42. 4. Chagnac A, Ben-Bassat M, Weinstein T, Levi J: Effect of long term Aluminium Administration of Renal Structure of Rats. Nephron, 1987; 47(1):66-69. 5. Ebina Y, Okade S, Hamazaki S, Midorikowa O: Liver, Kidney and Central nervous system toxicity of aluminium given intraperitoneally to rats: A multiple-dose subchronic study using aluminium nitritotriacetate. Toxicology & Applied Pharmacology, 1984;75(2):211- 218. 6. FAO/WHO: Aluminium. In : Evaluation of certain food additives and contaminants. (Thirtythird Report of the joint FAO/WHO Expert Committee on food additives.) WHO Technical Report Series, 1989; 776:26. 7. Garruto R M, Fukatsu R, Yanagihara R, Gajdusek D C, Hook G, Fiori C E: Imaging of Calcium and Aluminium in Neurofibrillary tangle - bearing neurons in Parkinsonism- Dementia of Guam. Proceedings of National Academic Sciences (USA), 1984; 81(6): 1875-1879. 8. Ghosh, M N: Procedures for rendering animals unconscious. In: Fundamentals of experimental phamacology. 2 nd Edn., Scientific Book Aency, 1984; pp 14. 9. Henry D A, Goodman WG, Nudelman RK, Didomenico ND, Alfrey A C , Slatopolsky E, Stanley T M, Coburn J W: Parenteral alumimium administration in the dog: 1. Plasma kinetics, tissue levels, Ca metabolism and parathyroid hormone. Kidney International, 1984; 25:362-369. 10. Neelam, Uday PK, Kaldhar M: Risk of Aluminium toxicity in Indian context. ICMR Buletin, 1999;29(8). 11. Perl D P: Relationship of Aluminium to Alzheimer's disease. Environmental Health Perspective, 1985;63: 149-153. 12. Shaver C G, Riddell A R: Lung changes associated with the manufactore of Alumina Abrasives. Journal of Industrial Hygiene & Toxicology, 1947; 29:145-157. 13. Smith D M: Aluminium containing dense deposit on glomerular membrane. American Journal of Clinical, Pathology, 1982;77:341-346. 14. Somova L 1, Missankov A, Khan MS: Chronic Aluminium intoxication in rats: Dose-dependent Morphological changes. Methodology & findings. Experimental Clinical Pharmacology, 1997; 19(9):599- 604. 15. Sorenson J R, Campbell I R, Tepper LB, Lingg RD: Aluminium in the environment and human health. Environmental Health Perspective, 1974; 8:3-95. 16. Spencer A J, Wood J A, Saunders H C, Freeman M S, Lote C J: Aluminium deposition in liver and kidney following acute intravenous administration of aluminium chloride or citrate in concious rats. Human Experimental Toxicology, 1995;14(10): 787-794."]}

Reports of aluminium toxicity on kidneys through dietary source is less reported even though it’s adverse effects such as encephalopathy, dementia, osteomalacia and fractures have been reported in renal failure cases after ingestion of aluminium gel. As Majority of Indian population is exposed to aluminium through dietary source, therefore, the effects of alumuinium toxiciy was studied in albino rats after oral administration of Aluminium salts. The present study was carried out on 40 albino rats divided into two equal group: control and experimental. Each of twenty animals of experimental group were given 37.5 mg of aluminium chloride per day orally for 3 weeks. All the animals were sacrificed on 22 nd day. Small pieces of kidney were procured, fixed in 10% neutral buffered formaline and embedded in paraffin. Sections of 7µ thickness were cut and stained with Haematoxylin and Eosin for general morphology and PAS for basement membrane. Significant histopathological changes were observed. Breakdown of general architectural pattern with degenerative changes were seen more in cortex as compared to medulla. Haemorrhage and inflammatory cell reaction was also observed in cortex.

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Keywords

Aluminium, Toxicity, Histopathology, Kidney.

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