Fig. 4. Quantification of dihydrocamalexic acid (DHCA) (m/z 247.0541, [C12H10N2O2S þ H]) in intercellular washing fluids (IWFs). DHCA levels measured in IWFs from Col-0, cyp71a12/cyp71a13, and cyp71b15 (all 7-weeks post-germination) 24 h after inoculation with P. syringae (Pst) or 10 mM MgCl2 (mock-inoculation) measured by UPLC-MS electrospray ionization in positive mode (ESI+). Values represent the mean ± standard deviation of three sample replicates (n = 3). Different letters indicate statistically significant differences (one-way ANOVA, Tukey's honestly significant difference [HSD], P <0.05). Standard curves were prepared using synthetic DHCA (2 pg–6 μg on column).

Published as part of Kempthorne, Christine J., Nielsen, Alexander J., Wilson, Daniel C., McNulty, James, Cameron, Robin K. & Liscombe, David K., 2021, Metabolite profiling reveals a role for intercellular dihydrocamalexic acid in the response of mature Arabidopsis thaliana to Pseudomonas syringae, pp. 1-13 in Phytochemistry (112747) 187 on page 6, DOI: 10.1016/j.phytochem.2021.112747, http://zenodo.org/record/8259052