Fig. 8. Effect of overexpression of different LjSK1 variants in L. japonicus hairy roots. Physiological parameters of L. japonicus wild type hairy roots transformed with LjSK1 variants: LjSK1 90–467 lacking the 89 N-terminal residues, LjSK1_K167A, LjSK1_Y298A, and native LjSK1 at 21 days post inoculation with rhizobium. Control roots were transformed with the empty T-DNA vector. Values shown are the average ± SEM of n = 6–16 individual hairy roots per construct. Letters above each graph indicate statistically significant differences (p <0.05) between samples using One-way ANOVA followed by Fischer's LSD post-hoc comparison.

Published as part of Solovou, Theodora G.A., Garagounis, Constantine, Kyriakis, Efthimios, Bobas, Charalambos, Papadopoulos, Georgios E., Skamnaki, Vassiliki T., Papadopoulou, Kalliope K. & Leonidas, Demetres D., 2021, Mutagenesis of a Lotus japonicus GSK3β/Shaggy-like kinase reveals functionally conserved regulatory residues, pp. 1-11 in Phytochemistry (112707) 186 on page 7, DOI: 10.1016/j.phytochem.2021.112707, http://zenodo.org/record/8258723