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Rural health in India depends a lot on the functioning of Sub-Centers. The conditions of the Sub-Centers in the country are far below than the expected performance. The present study aimed to find out the infrastructure and functioning of Sub-Centers, after introduction of National Rural Health Mission. Study design was cross-sectional. It followed multistage random sampling for selecting 16 Sub-Centers in Lucknow district, Uttar Pradesh. A score card was developed to evaluate different parameters in structure and functioning of Sub-Centers. Although labor room was present in half of the Sub- Centers, the deliveries were found to be conducted in none of those. Electricity and water supply were present only in 18.8% and 56.2% of the Sub-Centers respectively. Ante-natal registration and immunization were found to be good in 75% of the Sub-Centers. Post-natal care was virtually non-existing and referral of high risk pregnancies was very poor in most of the Sub-Centers. The focus of improvement should be centered on the betterment of structure and services provided at the Sub-Center level.