Single-cell and spatial transcriptomics of cardiac neural crest reveal a dual role of vinculin in Tgf beta signaling and cell-extracellular matrix interaction during cardiac outflow tract development

Single-cell and spatial transcriptomics of cardiac neural crest reveal a dual role of vinculin in Tgf beta signaling and cell-extracellular matrix interaction during cardiac outflow tract development. Single-cell RNA-seq data analysis: all.cncc.combined.EMBO.mapped.Rdata: public CNCC single-cell RNA-seq data integration E13.5_CNCC_merged_updated.Rdata: single-cell RNA-seq data of E13.5 CNCC generated in Elly lab all.seurat.GFP.Rdata: scRNA-seq data with GFP detected Spatial transcriptomics analysis: ST_seuratobj_merge: included 4 spatial slice samples merge object ST_seuratobj_Heart_region_subset: heart region of 4 spatial samples ST_seuratobj_Outflowtract_region_subset: OFT region of 4 spatial samples

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