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A cross sectional study was conducted to assess pre-analytical variables and sources of errors observed among hematology specimen received from IPD area in Central Pathology Laboratory of NKP Salve Institute of Medical Sciences & Research Centre and Lata Mangeshkar Hospital, Nagpur, Maharashtra during July 2016 - June 2017. Rate of sample rejection was noted to be highest from Orthopedics followed by Obstetrics and Gynecology, Medicine, Pediatric, Surgery, Eye, ENT, Pulmonary & Dermatology. The commonest cause for rejection was clotted samples. Other causes were hemolyzed sample, inadequate sample, excessive delay in sample transport and wrong patient identification. Faulty phlebotomy techniques, inappropriate preservation, improper transportation, wrong patient identification, lesser efficiency and carelessness were identified as other reasons.