Fig. 9. Cell cycle distribution and apoptosis induced by 8 in HepG2 cells. (A) Cell cycle analysis of PI-stained cells treated with different concentrations of 8 for 48 h by flow cytometry. (B) Apoptosis analysis of Annexin V-FITC/PI-stained cells treated with different concentrations of 8 for 48 h by flow cytometry. (C) Apoptosis analysis of Annexin V-FITC/PI-stained cells treated with 8 μM 8 for 6, 12, 24 and 48 h by flow cytometry. (D) Quantification of each stage of the cell cycle after treatment with 8. (E) Quantitative analysis of apoptotic cells treated with 3, 6 and 12 μM 8. (F) Quantitative analysis of apoptotic cells treated with 8 μM 8 for 6, 12, 24 and 48 h **P <0.01, ***P <0.001 versus control group.

Published as part of Jin, Yue, Wang, Miao, Yan, Yan-Fang, Zhang, Xin-Xin, Li, Xian-Zhe & Gao, Hui-Yuan, 2022, Bridged cassane derivatives from the seeds of Caesalpinia sappan L. and their cytotoxic activities, pp. 1-12 in Phytochemistry (113111) 197 on page 9, DOI: 10.1016/j.phytochem.2022.113111, http://zenodo.org/record/8236782