Background: Glaucoma is the second leading cause of untreatable blindness. Due to decreased hormonal levels in post-menopausal women as compared to the pre-menopausal state, there is a high risk of glaucoma in post-menopausal women. Also, women with high BMI and BP have higher chances of raised IOP. Aim: The study aims to compare intraocular pressure changes among pre-menopausal and post-menopausal women. Material and Methods: This was a cross-sectional study conducted for a period of 3 months from April to June 2023 on 140 women of age group 40-55 years who were divided into 2 groups each having 70 women: pre-menopausal and post-menopausal. The study was started after getting clearance from the institutional ethics committee. Women underwent thorough examinations which includes BMI, BP, and IOP. All values were taken and compared using STATA and SPSS software. Findings: The mean IOP in the pre-menopausal group was 11.75 mm of Hg and in the post-menopausal group was 13.42 mm of Hg which shows that in post-menopausal women the IOP is on the higher side and there are more chances of glaucoma in these women. The study has a p-value of 0.001. Conclusion: The study concludes that IOP in post-menopausal is on the higher side in comparison to pre-menopausal women. It is due to the reduced estrogen levels as compared to the pre-menopausal state. It was also seen that the women in both groups who have raised BP and BMI have increased IOP in comparison to women with normal BP and BMI.