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P. aeruginosa is a leading cause of nosocomial infections ranking second among the gram-negative pathogens. Hence this study was conducted to enhances the knowledge of this particular organism. A total of 100 isolates of P. aeruginosa isolated from various clinical specimens like urine, pus, blood, body fluids, sputum, collected from patients, irrespective of age and sex, were identified by standard microbiological procedures. Total hundred culture positive samples were taken and found that P. aerugenosa was predominantly present in urine sample of male aged between 21-30 years.