Background: A painful condition, rheumatoid arthritis frequently damages the patient's bones and joints. This autoimmune condition first affects tiny joints like the finger, thumb, toe, etc. The exact cause of the disease is unknown. These factors mostly include calcium shortage, elevated level of uric acid, poor fibre consumption, age, sex might cause arthritis. Women are more prominent to this disease as comparatively men.  Aim and Objectives: The aim of the study was to do the comparison between different nutrient deficiencies like calcium correlating with certain joints infections by determining RA factor.  Material And Methods: Three groups were generated from a total of 50 subjects in my research projects.29 abnormal, 14 control- normal, and 7 borderlines. The Quickem 200 Fully Auto Biochemistry Analyzer tested the patients' biochemistry by measuring their levels of calcium and RA factor. Calcium and RA factor are measured using the same Analyzer, and and the intensity of the colour is directly related to the concentration of the sample.Results: The results were expressed as Mean ± SD. The statistical analysis of the data was carried out with statistical package of social sciences(SPSS), version 20.0. The comparison between two groups were tested by ‘t’ test. In severe Rheumatoid Arthritis, the mean RA factor value were observed significantly high in all Age Groups i.e. (5-20 yrs.) (21-45 yrs.) and (45 yrs. Above) in both males and females. While calcium level decreases in all age groups in both males and females.Conclusion: The research study demonstrated as correlative association of Calcium with Rheumatoid arthritis. So, all these given parameters should be done during the treatment of Rheumatoid Arthritis and elevated level of calcium patient gives the results regarding infection in the blood due to inadequate removal of uric acid crystals as well as deficiency of Calcium which further can result in the low bone density. The above parameters should be determined during the diagnosis of RA in a patient.