Nanoparticles represent a promising drug delivery system of controlled and targeted drug release. They are specially designed to release the drug in the vicinity of target tissue. The aim of this study was to prepare and evaluate nanoparticles containing Isoniazid in different drug to polymer ratio by solvent evaporation technique. Prepared Nanoparticle was evaluated for its Particle Size, scanning electron microscopy, Percentage practical yield, Drug Entrapment Efficiency, and in-vitro drug release studies. FT-IR studies indicated that there was no chemical interaction between drug and polymer and stability of drug. The optimized Nanoparticles were found with particle size of 95.41 nm, percentage practical yield was 96.72 %. Entrapment efficiency (% EE) of 93.20 %, scanning electron microscopy irregular shape. The in-vitro release profile was found to be 98.19% sustained up to 12 h. The developed formulation overcome and alleviates the drawbacks and limitations of Isoniazid sustained release formulations and could possibility be advantageous in terms of increased bioavailability of Isoniazid. Keywords : Nanoparticles, Isoniazid and solvent evaporation technique