Fig. 2. Representative traces of currents elicited by using the agonist mode (a and c) and PAM mode (b and d) in the whole-cell mode of the patch-clamp technique at a holding potential of −70 mV in CHO cells stably expressing human α4β2 (a and b) and human α7 (c and d) nAChRs. Agonist mode (a, c): 0.3% DMSO (left); 33.3 μM of the tobacco alkaloid anabasine (middle); 33.3 and 900 μM ACh for α4β2 and α7, respectively (right). PAM mode (b, d): 0.4 and 100 μM ACh for α4β2 and α7, respectively (left); co-application of 33.3 μM of the tobacco alkaloid trans-anatalline (middle); 0.4 or 100 μM ACh for α4β2 and α7, respectively (right). Thick dotted lines represent the pre-application period of the alkaloid (≥2 min). Thin dotted lines represent zero current.; PAM, positive allosteric modulator.

Published as part of Alijevic, Omar, McHugh, Damian, Rufener, Lucien, Mazurov, Anatoly, Hoeng, Julia & Peitsch, Manuel, 2020, An electrophysiological characterization of naturally occurring tobacco alkaloids and their action on human α4β2 and α7 nicotinic acetylcholine receptors, pp. 1-20 in Phytochemistry (112187) 170 on page 9, DOI: 10.1016/j.phytochem.2019.112187, http://zenodo.org/record/8194187