Here we report SUPPORT (Statistically Unbiased Prediction utilizing sPatiOtempoRal information in imaging daTa), a self-supervised learning method for removing Poisson-Gaussian noise in voltage imaging data. SUPPORT is based on the insight that a pixel value in voltage imaging data is highly dependent on its spatially neighboring pixels in the same time frame, even when its temporally adjacent frames do not provide useful information for statistical prediction. Such spatiotemporal dependency is captured and utilized to accurately denoise voltage imaging data in which the existence of the action potential in a time frame cannot be inferred by the information in other frames. Through simulation and experiments, we show that SUPPORT enables precise denoising of voltage imaging data while preserving the underlying dynamics in the scene. We also show that SUPPORT can be used for denoising time-lapse fluorescence microscopy images of Caenorhabditis elegans (C. elegans), in which the imaging speed is not faster than the locomotion of the worm, as well as static volumetric images of Penicillium and mouse embryos. SUPPORT is exceptionally compelling for denoising voltage imaging and time-lapse imaging data, and is even effective for denoising calcium imaging data. For more details, please see the accompanying research publication "Statistically unbiased prediction enables accurate denoising of voltage imaging data". Datasets for publication titled "Statistically unbiased prediction enables accurate denoising of voltage imaging data" Voltage imaging of paQuasAr6a: paQuasAr6a.zip paQuasAr6a/Q6a_Cell1 paQuasAr6a/Q6a_Cell2 paQuasAr6a/Q6a_Cell3 paQuasAr6a/Q6a_Cell4 paQuasAr6a/Q6a_Cell5 paQuasAr6a/Q6a_Cell6 paQuasAr6a/175118PP046_P8_pulse (10 ms)_q6 paQuasAr6a/181625PP046_P5_q6_pulse(50ms) paQuasAr6a/183616PP046_P8_pulse (10 ms)_q6 paQuasAr6a/183717PP046_P5_q6_pulse(50ms) Voltage imaging of Voltron2: Voltron2.zip Voltron2/Voltron_Cell1 Voltron2/Voltron_Cell2 Voltron2/Voltron_Cell3 Voltron2/Voltron_Cell3_2 Voltron2/Voltron_Cell4 Voltron2/Voltron_Cell5 Voltron2/Voltron_Cell6 Voltron2/Voltron_Cell7 (100f_s) Voltron2/Voltron_Cell7_2 (100 f_s) Voltage imaging of zArchon (zebrafish spinal cord): zebrafish_spinal_cord_N.zip Voltage imaging of SomArchon (mouse hippocampus neuron): SomArchon.zip Volumetric structural imaging of mouse embryo (Expansion microscopy): Expansion_microscopy.zip Expansion_microscopy/JEME208_2x_expanded_bone.tif Expansion_microscopy/JEME208_2x_expanded_intenstine.tif Expansion_microscopy/JEME209_2x_expanded_bone_1.tif Expansion_microscopy/JEME209_2x_expanded_bone_2.tif Expansion_microscopy/JEME209_2x_expanded_tail.tif Volumetric structural imaging of penicillium: Penicillium.zip Penicillium/penicillium_low_snr.tif --> Low SNR image Penicillium/penicillium_high_snr.tif --> High SNR image Calcium imaging of zebrafish: Zebrafish.zip Zebrafish/zebrafish_multiple_brain_regions.tif --> Multiple brain regions Zebrafish/zebrafish_Cerebellar_plate.tif --> Cerebellar plate Zebrafish/zebrafish_Dorsal_telencephalon.tif --> Dorsal telencephalon Zebrafish/zebrafish_Medulla_oblongata.tif --> Medulla oblongata Zebrafish/zebrafish_Olfactory_bulb.tif --> Olfactory bulb Zebrafish/zebrafish_Optic_tectum.tif --> Optic tectum Zebrafish/zebrafish_Habenula.tif --> Habenula