The present study was undertaken to investigate in vitro antioxidant activity of aqueous and hydroalcoholic extract of whole plant of Tinospora Cordifolia. Family- Scrophularaceae. The total Phenolic content was determined using folin ciocalteau method while the total flavanoid content was determined using aluminum chloride method. In vitro antioxidant activity was evaluated using the Reducing power assay , Hydrogen peroxide scavenging assay, nitric oxide scavenging activity, superoxide scavenging activity and hydroxyl radical scavenging activity. The hydroalcoholic extract had more phenol concentration ( 116.1 mg/g of extract ) when compared to aqueous extract ( 58 mg/g of extract ). The flavanoid content was more in hydroalcoholic extract ( 242.6 mg/g of extract ) when compared to that of aqueous extract ( 202.8 mg/g of extract ). The reducing power and hydrogen peroxide scavenging of the extract was found to be concentration dependent. The nitric oxide scavenging activity . superoxide scavenging activity and Hydroxyl radical scavenging activity was also concentration dependent with IC50 value being 254.70 µg/ml , 934.06 µg/ml and 510.60 µg/ml respectively for Aqueous extract and 169.22 µg/ml , 495.83 µg/ml , 488 µg/ml respectively for hydroalcoholic extract. The order of the antioxidant potency of the whole plant extract is Hydroalcoholic >> aqueous. The results clearly indicate that aqueous and hydroalcoholic extract of Tinospora Cordifolia. has anti oxidant property which may be due to the presence of phenols and flavanoids. Keywords: Tinospora Cordifolia. ,Superoxide Scavenging Activity,Hydroxyl Radical Scavenging Activity,Hydrogen Peroxide Scavenging Activity .